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Reclamation of Mine-Impacted Land for Ecosystem Recovery
by : Nimisha Tripathi, Raj Shekhar Singh, Colin D. Hills
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Praktik Pengelolaan Limbah Padat dan B3 (Bahan Berbahaya dan Beracun)
by : Arief Sabdo Yuwono, Wiranda Intan Suri, Elsy Gustika Buana, Rosidana, Afu Vianti Driantika, Priscilia Dana Mentari, Agung Abdul Raup, Nanda Savira Ersa
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The possibility of controlling Sclerotium rolfsii on soybean Glycine max using Trichoderma and Tebucanazole
Researcher : O.S. Dharmaputra, I. Retnowati
Published : 1994
Group :Microorganism
Full Report : Special request
Abstract :
The possibility of controlling S.Rolfsii on soybean (Glycine max) var. Rinjani using T. aureoviridi and Tebuconazole under field conditions was studied. The experiment was conducted at the experimental plot of SEAMEO BIOTROP.
The pathogen was mixed with the soil (2 kg/plot) 4 days before the inoculation of the antagonist (2.25 kg/plot). The measurement of each plot was 2.5 x 6 m. N,P and K (120 kg/ha) were applied at the same day with the inoculation of the pathogen. Soybean seeds were planted 7 days after the inoculation of the antagonist. The distance between plants and between plots were 20 and 40 cm, respectively. The fungicide at concentration of 100 g/ha (in vitro concentration) and 210 g/ha (field or recommended concentration) were applied using 2 methods, i.e. 1) spraying on the planting hole at the same day as the planting of soybean seeds, and 2) spraying on the soil surrounding the plants 7 days after planting. Soils that were neither inoculated with the antagonist nor the fungicide were used as controls. Three applications (3 plots) were used for each treatment (including the control).

The result showed that the inoculation of the antagonist, the concentrations of the fungicide, and the time of application gave very significant differences in the precentages of the plants infected by the pathogen and significant differences in seed production; while the interaction between the inoculation of the antagonist and the concentrations of the fungicide, between the concentrations of the fungicide and the time of application, and between the inoculation of the antagonist, the concentration of the fungicide and the time of application did not give significant differences either the precentages of the plants infected by the pathogen or seed production.

The precentage of plants infected by the pathogen was lower on soil inoculated with the antagonist (31.6%) than on soil not inoculated with the antagonist (52.9%).The precentage of plants infected by the pathogen was lower on soil treated with the fungicide either at in vitro concentration (37.5%) or an field concentration (37.4%) than on the soil not treated with the fungicide (61.5%). Nevertheless, based on statistical analysis, the fungicide at in vitro concentration was not significantly different from that at field concentration. ; The precentage of the plants infected by the pathogen on the soil sprayed with fungicide at the same day of seed planting was lower (30.5%) than sprayed 7 days after planting (44.$%).
The seed production on the soil inoculated with the antagonist (1893.3 kg/ha) was higher than on the soil not inoculated with the antagonist (1465.7 kg/ha).

The production on the soil sprayed with the fungicide at in vitro (1758.0 kg/ha) or at field concentration (1817.1 kg/ha) was higher than on the soil not sprayed with the fungicide (1247.2 kg/ha). The production on the soil sprayed with the fungicide at the same day of the planting (2010.9 kg/ha) was higher than sprayed 7 days after planting (1564.2 kg/ha).

The combination between the inoculation of the antagonist and the fungicide application at in vitro concentration at the same day of seed planting gave higher seed production (2391.2 kg/ha) than the inoculation of the antagonist (1711.7 kg/ha) or the fungicide application either at in vitro concentration  (1771.9 kg/ha) or at field concentration (1939.1 kg/ ha) at the same day of seed planting. However, based on statistical analysis, the interaction  among the three treatments (the antagonist, the concentrations of the fungicide, and the time of application) was not significantly different.