Book Review
Reclamation of Mine-Impacted Land for Ecosystem Recovery
by : Nimisha Tripathi, Raj Shekhar Singh, Colin D. Hills
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Update: 13 Jul 2018
Published: since 1 Mar 2008

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The Effectiveness of Various Formulation of Endophytic Bacteria from Mangrove to Control Phytophthora Leaf Blight on Japanese Taro
Researcher : A. Munif, M. F. Oktafiyanto, D. D. Eris
Published : 2017
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Abstract :

The development of Japanese taro in Indonesia is faced on many problems. One of the problems in the cultivation of taro satoimo is leaf blight disease caused by Phytophtora colocasiae. The pathogen is capable in producing spores that are ready to be released in water and spread through water splashes, usually it will increase during the rainy season. The decrease of taro tuber production caused by Phytophtora colocasiae attack can reach up to 50%, this obviously causing economically high losses. The objective of this research was to know the potential of various formulations in maintaining viability of selected endophytic bacteria from mangrove, their potency in suppressing the growth of Phytophtora colocasiae and to identity of endophytic bacteria from selected mangrove plant with molecular technique.

The research method includes pathogen isolation, selected endophytic bacteria formulation, formulation stability test, application of formulation on plant and observation of disease occurrence and severity and identification of bacterial isolates molecularly. The result showed based on the research it is known that compost formulation has the best stability capacity compared to other formulations in maintaining viability of endophytic mangrove bacteria up to sixteen weeks. Data shown that mixture endophytic bacteria treatment is better than single isolate treatment.

In the test of liquid formulation, the severity and incidence of isolate A treatment is the treatment which shows the best disease severity suppression value up to 57% followed by ABC mixture bacteria treatment which can suppress disease severity up to 35%. In the test of dust/flour formulation and solid/compost formulation the value of severity and incidence of ABC isolate treatment has showed as the best disease severity suppression indicated by the lowest severity and disease occurrence of 20% (KeP and KjP dust/flour formulation) and 6.6% and 20% (KeP and KjP solid/compost formulations). It was also found that YA1K5-7 isolates in dust/flour formulation showed the highest plant height and root weight of 68.4 cm and 44.9 gr. While the isolates of BR1K5-4 in solid/compost formulation have the best fresh weight of 94.8 gr. In this study, ten selected isolates of endophytes mangrove have been identified by molecular technique as Bacterium NLAE-zl-H299, Providencia rettgeri, Providencia vermicola, Enterococcus faecalis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus thuringiensis. All of them has the ability to regulate plant growth and suppress the development of pathogen.

Keywords: Mangrove, Endophytic Bacteria, Phytophthora colocasiae, Plant Disease Control, Bacterial Formulation