Book Review
Reclamation of Mine-Impacted Land for Ecosystem Recovery
by : Nimisha Tripathi, Raj Shekhar Singh, Colin D. Hills
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Update: 13 Sep 2019
Published: since 1 Mar 2008

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Acclimatization and Field Cultivation of Regenerated Cottonii Seaweed (Kappaphycus alvarezii Doty) from Tissue Culture in coastal waters.
Researcher : E. Sulistiani, D. T. Soelistyowati, S. A. Yani, H.Affandi


Published : 2012
Group :Agriculture
Full Report : Special request
Abstract :
Young thallus or plantlet of Cottonii seaweed ready to acclimatize had been obtained through tissue culture technique at SEAMEO BIOTROP. Therefore further studies are needed to learn how to acclimatize and do cultivation trial of these plantlets in coastal waters to verify their survivability and growth, also the carrageenan content of harvested seaweed derived from tissue culture. The plantlets were acclimatized in aquarium with seawater recirculation system. Acclimatization was conducted in three fertilizer treatments namely, 1.2 mg/l of inorganic fertilizer, 0.25 ml/l of organic fertilizer, 5 ml/l of PES (Provasoli enrichment solution) and seawater without addition of fertilizer as a control. Cultivation trials of tissue culture seaweeds were conducted in two location, namely at Hanura coastal water, Lampung Province and at Gerupuk Bay, Lombok Island, West Nusa Tenggara Province. The result of cultivation trials in Lampung indicated that fertilizer treatment during acclimatization significantly affected daily growth rate (DGR) and survival rate (SR) of tissue culture seaweed when grown in coastal waters (p<0.05). After 3 weeks of planting, tissue culture seaweeds derived from acclimatization without fertilizer had DGR (13.46 %) higher than seaweeds derived from acclimatization with fertilizer (10.39-12.20%). However, the tissue culture seaweeds from all fertilizer treatment had DGR higher than seaweeds derived from cutting (7.62%). Until 4 weeks of planting, the SR of tissue culture seaweeds derived from acclimatization without fertilizer (85%) was higher than SR of seaweeds derived from acclimatization with fertilizer (45-75%). While in seaweeds derived from cutting, 100% were still alive until 4 weeks of planting. The cultivation trial in Lombok Island used tissue culture seaweeds were acclimatized without fertilizer, but seawater was changed every 2 weeks. The SR of these tissue culture seaweeds when cultivated in coastal water was 96.7%. The tissue culture seaweeds also had DGR (11.5%) higher than seaweeds from cutting (5.75 - 7.48%) after 4 weeks of planting. Harvested seaweeds (aged 35 days of planting) from the cultivation trials in Lampung and Lombok were analyzed for the carrageenan content. The results indicated that seaweeds derived from tissue culture had carrageenan content higher (40.7-44.1%) than seaweeds derived from cutting (33.0-36.9%).